<i>B. burgdorferi</i> labeled with BODIPY-cholesterol release the fluorescent cholesterol probe in OMV.

<p><b>A.</b> BODIPY-cholesterol labeled and unlabeled <i>B. burgdorferi</i> release similar amounts of membrane material. DPH was added to the supernatants collected from <i>B. burgdorferi</i> labeled with BODIPY-cholesterol and unlabeled spirochetes to measure released OMV. Using the SpectraMaxM2, the DPH fluorescence was calculated for each sample at the specific time points. Supernatant from labeled <i>B. burgdorferi</i> (black); supernatant from unlabeled <i>B. burgdorferi</i> (gray). <b>B.</b> Transmission electron micrograph showing isolated vesicles from both labeled and unlabeled <i>B. burgdorferi</i> OMV. Immunogold labeling of OspB (18 nm) and <i>B. burgdorferi</i> glycolipids (6 nm) is seen throughout the vesicles. Scale Bar = 100 nM. Experiment <b>A</b> represents the mean ± standard error of the mean from three separate experiments.