<p>Vesicles purified from <i>B. burgdorferi</i> labeled with BODIPY-cholesterol were incubated with adherent HeLa cells for 2 hrs. <b>A.</b> Vybrant Cell Labeling Solution DiI labels the plasma membrane of HeLa cells. <b>B.</b> Single image of OMV labeled with BODIPY-cholesterol and Vybrant Cell Label Solution DiI. Incubation of labeled OMV (green) colocalize (yellow) at the plasma membrane (red). <b>C.</b> Merged three dimensional confocal micrograph demonstrate Vybrant Cell Labeling Solution DiI (red) is located at the plasma membrane of the HeLa cell. There is colocalization (yellow) of labeled OMV (green) on the surface of the HeLa cells. Scale Bar = 20 µm.</p
Low-dosage ultraviolet confocal fluorescence microscopy and filipin staining showed that cholesterol...
SummaryBorrelia burgdorferi, the agent of Lyme disease, is unusual as it contains free cholesterol a...
International audienceEnterohemorrhagic Escherichia coli (EHEC) O157:H7 is a major cause of foodborn...
<p><b>A.</b> BODIPY-cholesterol labeled and unlabeled <i>B. burgdorferi</i> release similar amounts ...
<p>HeLa cells incubated with BODIPY-cholesterol (green) and washed with MβCD were incubated with <i>...
<p><i>B. burgdorferi</i> labeled with BODIPY-cholesterol were incubated with HeLa cells for 15 min, ...
<p>OMV from labeled and unlabeled <i>B. burgdorferi</i> were purified using an Optiprep density grad...
<p><b>A.. </b><i>B. burgdorferi</i> utilized <sup>3</sup>H-cholesterol as a substrate for cholestero...
<div><p><em>Borrelia burgdorferi</em>, the agent of Lyme disease, has cholesterol and cholesterol-gl...
<p>AGS cells labeled with green CellTrackerTM were incubated for 4 hours with HBSS (Figure 2A) or <i...
Borrelia burgdorferi, the agent of Lyme disease, has cholesterol and cholesterol-glycolipids that ar...
<p>Localization of rhodamine B-R18-labeled MVs (red fluorescence used as a readout for MV fusion wit...
Borrelia burgdorferi, the agent of Lyme disease, has cholesterol and cholesterol-glycolipids that ar...
<div><p>Lipid rafts in eukaryotic cells are sphingolipid and cholesterol-rich, ordered membrane regi...
<p>A. and B. In contrast to the wild type cells in which BodipyC12 labels host LDs (A), the fluoresc...
Low-dosage ultraviolet confocal fluorescence microscopy and filipin staining showed that cholesterol...
SummaryBorrelia burgdorferi, the agent of Lyme disease, is unusual as it contains free cholesterol a...
International audienceEnterohemorrhagic Escherichia coli (EHEC) O157:H7 is a major cause of foodborn...
<p><b>A.</b> BODIPY-cholesterol labeled and unlabeled <i>B. burgdorferi</i> release similar amounts ...
<p>HeLa cells incubated with BODIPY-cholesterol (green) and washed with MβCD were incubated with <i>...
<p><i>B. burgdorferi</i> labeled with BODIPY-cholesterol were incubated with HeLa cells for 15 min, ...
<p>OMV from labeled and unlabeled <i>B. burgdorferi</i> were purified using an Optiprep density grad...
<p><b>A.. </b><i>B. burgdorferi</i> utilized <sup>3</sup>H-cholesterol as a substrate for cholestero...
<div><p><em>Borrelia burgdorferi</em>, the agent of Lyme disease, has cholesterol and cholesterol-gl...
<p>AGS cells labeled with green CellTrackerTM were incubated for 4 hours with HBSS (Figure 2A) or <i...
Borrelia burgdorferi, the agent of Lyme disease, has cholesterol and cholesterol-glycolipids that ar...
<p>Localization of rhodamine B-R18-labeled MVs (red fluorescence used as a readout for MV fusion wit...
Borrelia burgdorferi, the agent of Lyme disease, has cholesterol and cholesterol-glycolipids that ar...
<div><p>Lipid rafts in eukaryotic cells are sphingolipid and cholesterol-rich, ordered membrane regi...
<p>A. and B. In contrast to the wild type cells in which BodipyC12 labels host LDs (A), the fluoresc...
Low-dosage ultraviolet confocal fluorescence microscopy and filipin staining showed that cholesterol...
SummaryBorrelia burgdorferi, the agent of Lyme disease, is unusual as it contains free cholesterol a...
International audienceEnterohemorrhagic Escherichia coli (EHEC) O157:H7 is a major cause of foodborn...