bDHC induces cell cycle impairment and apoptosis in HCT116 cells.

<p><b>A.</b> Left panel: Structure of bDHC. Right panel: Dose-response effect of bDHC on cell viability upon 24 hours treatment, compared to control and DMSO-treated cells. <b>B.</b> PI/FACS analysis of cell cycle progression after DMSO, Curcumin (10 µM) and bDHC (30 µM) treatments for 16 and 24 hours (left and right panel, respectively). The indicated events are means of ten independent experiments (A = SubG1, B = G0/G1, C = S, D = G2/M). <b>C.</b> PI/BrdU bivariate FACS analysis of 16 and 24 hours treatments with DMSO, Curcumin and bDHC. Analysis was gated to exclude SubG1 population. <b>D.</b> Left panel: The percentage of Annexin V positive cells upon 24 hours treatment with bDHC is compared to DMSO- and Curcumin-treated cells. Data are means of three independent experiments −/+ SD. Middle panel: PI/monoparametric cell cycle analysis of bDHC-treated cells <i>versus</i> bDHC-cells released for 24 hours in fresh medium. Events are indicated as means of three independent experiments (A = SubG1, B = G0/G1, C = S, D = G2/M). Right panel: γ-H2AX expression levels <i>versus</i> actin in cells treated with bDHC for 24 hours.