Protein-protein interactions of zebrafish family of Ssat1 proteins.

<p>(A) GST, GST-Ssat1a, GST-Ssat1b, and GST-Ssat1c were purified from bacteria. Lysates of cells transfected with the plasmid encoding Ssat1b were incubated with GST (Lane 1) or GST-Ssat1a (Lane 2). Lysates of cells transfected with the plasmid encoding Ssat1c were incubated with GST (Lane 3), GST-Ssat1a (Lane 4), or GST-Ssat1b (Lane 5). (B) GST and GST fused with the cytosolic domain of zebrafish Integrin 9α (GST-Intg9α_c) were purified from bacteria. Lysates of cells transfected with the plasmid encoding Ssat1a (lanes 1 and 2), Ssat1b (lanes 3 and 4), or Ssat1c (lanes 5 and 6) were incubated with GST (lanes 1, 3 and 5) or GST-Intg9α_c (lanes 2, 4 and 6). (C) Lysates of cells transfected with the plasmid encoding the myc-tagged PAS-B domain of Zebrafish Hif-1α was incubated with GST (lane 1), GST-Ssat1a (lane 2), GST-Ssat1b (lane 3), or GST-Ssat1c (lane 4). Bound proteins in each sample were pulled down with glutathione Sepharose 4B beads and analyzed by western blotting.