Overexpression of Prox1 results in the expression of lymphatic markers on the jugular vein.

<p>(A) Normally, the expression of Podoplanin (FITC) on the jugular vein is downregulated by E13.5 and upregulated in lymph sacs, along with Prox1 (Cy3). (B) Prox1 overexpression results in its' expression on the jugular vein as well as the lymph sac. Furthermore, Podoplanin is now found expressed on the jugular vein (arrows). Note that the lymph sac has become significantly enlarged. Similarly, immunohistochemistry on (C) control and (D) double transgenic E13.5 embryos show an increase in staining of LYVE-1 (arrows) on the lymph sac and jugular vein. Scale bar = 25 µm. JV: jugular vein; LS: lymph sac.