Expression pattern of <i>HXK1</i> in filamentation inducing media.

<p>A) Hxk1 transcript levels are upregulated in response to filamentation conditions in wild type and mutants for filamentation regulators. Wild-type strain and mutants were grown in YPD for 6 hr at 30°, washed, starved for 10 hrs and induced in YPD, GlcNAc, Spider and Serum (Experimental procedures). Cells were harvested at 2 hrs time point in case of YPD, Spider or Serum and at 4 hrs time point in case of GlcNAc and the total RNA was prepared and northern blot analyses were carried out. Ribosomal (r RNA) has been included as loading control. B) Quantitative RT-PCR of <i>HXK1</i> basal transcript levels for the wild type and mutant cells grown in YPD. Almost unaltered HXK1 mRNA levels were observed in all strains. Error bars represent the coefficient of variation (n = 3). C) Northern blot analysis for the time kinetics of <i>HXK1.</i> Transscript levels are in response to Spider at 37°C. Ribosomal (r RNA) has been included as loading control. Corresponding cell shots representing hyperfilamentous morphology of <i>hxk1</i> mutant in comparison with wild type strain have also been shown at the bottom.