<p>Cells co-transfected with Yip1A siRNA and HA-Yip1A Δ1-83 were fixed after 72 h and co-stained with antibodies against HA (A) and calnexin (B). The asterisk (A and B) marks an expressing cell that did not exhibit ER whorls. Scale bar, 10 μm. (C) Quantification of the efficiency of rescue by HA-Yip1A Δ1–83, HA-Yip1A Δ1–118 and the negative control Myc-Sec61β. Data were from 3 independent experiments (>100 cells per experiment), ±SD. (D) An alignment of the cytoplasmic domains of human Yip1A with yeast Yip1p. Residues 83–118 are bracketed, with the highly conserved block highlighted in bold. (E) Bolded residues (in D) were mutated as indicated and tested for rescue. Data from 3 independent experiments (>100 cells per experiment) ±SD are qua...
<div><p>A common virulence mechanism among bacterial pathogens is the use of specialized secretion s...
A common virulence mechanism among bacterial pathogens is the use of specialized secretion systems t...
To establish an infection, Yersinia pseudotuberculosis utilizes a plasmid-encoded type III transloco...
<p>(A) Quantification of cells that were co-transfected with the indicated HA-Yip1A mutated construc...
<p>HeLa cells were co-transfected with Yip1A siRNA and either a negative control myc-Sec61β construc...
<p>(A) Quantification of rescue in cells that were co-transfected with Yip1A siRNA and mutated HA-Yi...
The endoplasmic reticulum (ER) of specialized cells can undergo dramatic changes in structural organ...
The endoplasmic reticulum (ER) of specialized cells can undergo dramatic changes in structural organ...
<p>HeLa cells were transfected with each siRNA for 24 hr, and then treated with Tm for 5 hr to induc...
The mammalian endoplasmic reticulum (ER) is the largest organelle in the cell, extending from the nu...
<p>HeLa cells were transfected with each siRNA for 24 hr, and then treated with Tm to induce the UPR...
(A) Yeast lacking selected Hsp70 co-chaperones are sensitive to HU. WT BY4742 or BY4742 cells lackin...
<p>HeLa cells were infected with <i>B</i>. <i>abortus</i>, and then transfected with each siRNA at 1...
<p>Wild-type Yki is 418 amino acids long, whereas Yki-ΔC lacks the final 51 amino acids at the C-ter...
Type III secretion systems (T3SSs) are tightly regulated key virulence mechanisms shared by many Gra...
<div><p>A common virulence mechanism among bacterial pathogens is the use of specialized secretion s...
A common virulence mechanism among bacterial pathogens is the use of specialized secretion systems t...
To establish an infection, Yersinia pseudotuberculosis utilizes a plasmid-encoded type III transloco...
<p>(A) Quantification of cells that were co-transfected with the indicated HA-Yip1A mutated construc...
<p>HeLa cells were co-transfected with Yip1A siRNA and either a negative control myc-Sec61β construc...
<p>(A) Quantification of rescue in cells that were co-transfected with Yip1A siRNA and mutated HA-Yi...
The endoplasmic reticulum (ER) of specialized cells can undergo dramatic changes in structural organ...
The endoplasmic reticulum (ER) of specialized cells can undergo dramatic changes in structural organ...
<p>HeLa cells were transfected with each siRNA for 24 hr, and then treated with Tm for 5 hr to induc...
The mammalian endoplasmic reticulum (ER) is the largest organelle in the cell, extending from the nu...
<p>HeLa cells were transfected with each siRNA for 24 hr, and then treated with Tm to induce the UPR...
(A) Yeast lacking selected Hsp70 co-chaperones are sensitive to HU. WT BY4742 or BY4742 cells lackin...
<p>HeLa cells were infected with <i>B</i>. <i>abortus</i>, and then transfected with each siRNA at 1...
<p>Wild-type Yki is 418 amino acids long, whereas Yki-ΔC lacks the final 51 amino acids at the C-ter...
Type III secretion systems (T3SSs) are tightly regulated key virulence mechanisms shared by many Gra...
<div><p>A common virulence mechanism among bacterial pathogens is the use of specialized secretion s...
A common virulence mechanism among bacterial pathogens is the use of specialized secretion systems t...
To establish an infection, Yersinia pseudotuberculosis utilizes a plasmid-encoded type III transloco...