<p><b>A.</b> Glycans released from either HeLa cells embedded in polyacrylamide gels (H) or HeLa cell lysates (HL) were labeled with 2-AB and separated by HPLC. The histogram shows the partition (percentage + SD) of individual glycan fractions (GP1 – GP12) of HPLC separated glycome. <b>B.</b> Coefficients of variation (%CV) of individual glycan peaks in HPLC separated glycome of HeLa cells embedded in polyacrylamide gels (H) and HeLa cell lysates (HL) from 6 identical experiments.</p
<p>Quantified GSL-glycans were stratified into ganglio (Gg), globo (Gb), (neo)lacto (n)Lc, “Gg or (n...
<p>Equal amounts of whole cellular protein lysates (7.5 μg) derived from native (N) and cultured, my...
Percentage of different glycan species on gp120 produced by CHO-S and MGAT1− CHO cells.</p
<p>The resulting 35 peaks were divided into Group 1 (Peak 1–12), Group 2 (Peak 13–23), Group 3 (Peak...
1<p>GU value represents the peak elution as compared to that of 2-aminobenzamide-labelled oligomers ...
<p>Confocal images of HeLa cells embedded in polyacrylamide gel. The peripheral cellular glycans are...
<p>The small vertical bars below the 0 level denote the integration area for each peak, and the majo...
<p>(a) Elution profile of DEAE anion-exchange-chromatography. PA-<i>N</i>-glycans from human serum w...
<p>(A) MALDI-TOF MS spectra of N-glycans released from membrane protein of Hca-F and Hca-P cell line...
<p>The data were shown as molar percentage. The presented numbers were average of two analyses.</p><...
<p>The desialylated N-glycan profiles of membrane proteins from cell lines (n=8) and tumor tissues (...
<p>Purified pHpt-N, pHpt-P, and sHpt-P were deglycosylated by treatment with PNGase F, and their gly...
The cell membrane plays an important role in protecting the cell from its extracellular environment....
Although many of the frequently used pluripotency biomarkers are glycoconjugates, a glycoconjugate-b...
<p>Each set of data was collected from 1 mg of sample. The presented numbers were average of two ana...
<p>Quantified GSL-glycans were stratified into ganglio (Gg), globo (Gb), (neo)lacto (n)Lc, “Gg or (n...
<p>Equal amounts of whole cellular protein lysates (7.5 μg) derived from native (N) and cultured, my...
Percentage of different glycan species on gp120 produced by CHO-S and MGAT1− CHO cells.</p
<p>The resulting 35 peaks were divided into Group 1 (Peak 1–12), Group 2 (Peak 13–23), Group 3 (Peak...
1<p>GU value represents the peak elution as compared to that of 2-aminobenzamide-labelled oligomers ...
<p>Confocal images of HeLa cells embedded in polyacrylamide gel. The peripheral cellular glycans are...
<p>The small vertical bars below the 0 level denote the integration area for each peak, and the majo...
<p>(a) Elution profile of DEAE anion-exchange-chromatography. PA-<i>N</i>-glycans from human serum w...
<p>(A) MALDI-TOF MS spectra of N-glycans released from membrane protein of Hca-F and Hca-P cell line...
<p>The data were shown as molar percentage. The presented numbers were average of two analyses.</p><...
<p>The desialylated N-glycan profiles of membrane proteins from cell lines (n=8) and tumor tissues (...
<p>Purified pHpt-N, pHpt-P, and sHpt-P were deglycosylated by treatment with PNGase F, and their gly...
The cell membrane plays an important role in protecting the cell from its extracellular environment....
Although many of the frequently used pluripotency biomarkers are glycoconjugates, a glycoconjugate-b...
<p>Each set of data was collected from 1 mg of sample. The presented numbers were average of two ana...
<p>Quantified GSL-glycans were stratified into ganglio (Gg), globo (Gb), (neo)lacto (n)Lc, “Gg or (n...
<p>Equal amounts of whole cellular protein lysates (7.5 μg) derived from native (N) and cultured, my...
Percentage of different glycan species on gp120 produced by CHO-S and MGAT1− CHO cells.</p