<p>Surface expression of CD3, CD4, CD8 and CD19 were measured by flow cytometry; the relative fluorescence intensities are indicated as a percentage of positive cells. The results shown are the mean±SE of three independent experiments.</p
<p>Thymocytes were stained with fluorochrome-conjugated anti-CD3, anti-CD4 and anti-CD8 mAbs, and su...
<p>Cells were stained with a combination of antibodies commonly used for the analysis of immune cell...
<p>(<b>A</b>) Cell suspensions from healthy and tumoral tissues obtained from mice of the indicated ...
<p>Splenocytes were isolated from mice and labeled as described in the <a href="http://www.plosone.o...
<p>Flow cytometry graphs showing the cell populations in spleen from one representative mouse from e...
<p>(A) Thymocytes were stained with PE-conjugated anti-CD4, CyChrome-conjugated anti-CD8, and FITC-c...
Plots are gated on CD3 cells (A) or GFP CD3 cells (B). Numbers indicate mean percent cells in quadra...
<p><i>A. Weight and cellularity of WT and KO thymi and spleens.</i> Mouse number (n) in each group i...
<p>A. T cell sub-populations in the spleen and LN. CD4 and CD8 T cell populations in the WT and Tg s...
<p>Thymocytes were isolated from <i>Ptch<sup>flox/flox</sup></i> control or <i>Ptch<sup>flox/flox</s...
<p>A. T cell subpopulations in the spleen and LN. CD4 and CD8 T-cell populations in WT and HET splee...
<p>Lymph node lymphocytes (A), thymocytes (B), splenocytes (C and D) or bone marrow cells (E and F) ...
<p><b>A:</b> Activated CD4 T cells in the spleens of mice (n = 9), represented as a percentage of ce...
<p>Flow cytometry was performed on the peripheral blood cells, splenocytes, and thymocytes from RasG...
<p>Total cell counts of splenocytes and splenic B cells (B220<sup>+</sup> gated lymphocytes) from WT...
<p>Thymocytes were stained with fluorochrome-conjugated anti-CD3, anti-CD4 and anti-CD8 mAbs, and su...
<p>Cells were stained with a combination of antibodies commonly used for the analysis of immune cell...
<p>(<b>A</b>) Cell suspensions from healthy and tumoral tissues obtained from mice of the indicated ...
<p>Splenocytes were isolated from mice and labeled as described in the <a href="http://www.plosone.o...
<p>Flow cytometry graphs showing the cell populations in spleen from one representative mouse from e...
<p>(A) Thymocytes were stained with PE-conjugated anti-CD4, CyChrome-conjugated anti-CD8, and FITC-c...
Plots are gated on CD3 cells (A) or GFP CD3 cells (B). Numbers indicate mean percent cells in quadra...
<p><i>A. Weight and cellularity of WT and KO thymi and spleens.</i> Mouse number (n) in each group i...
<p>A. T cell sub-populations in the spleen and LN. CD4 and CD8 T cell populations in the WT and Tg s...
<p>Thymocytes were isolated from <i>Ptch<sup>flox/flox</sup></i> control or <i>Ptch<sup>flox/flox</s...
<p>A. T cell subpopulations in the spleen and LN. CD4 and CD8 T-cell populations in WT and HET splee...
<p>Lymph node lymphocytes (A), thymocytes (B), splenocytes (C and D) or bone marrow cells (E and F) ...
<p><b>A:</b> Activated CD4 T cells in the spleens of mice (n = 9), represented as a percentage of ce...
<p>Flow cytometry was performed on the peripheral blood cells, splenocytes, and thymocytes from RasG...
<p>Total cell counts of splenocytes and splenic B cells (B220<sup>+</sup> gated lymphocytes) from WT...
<p>Thymocytes were stained with fluorochrome-conjugated anti-CD3, anti-CD4 and anti-CD8 mAbs, and su...
<p>Cells were stained with a combination of antibodies commonly used for the analysis of immune cell...
<p>(<b>A</b>) Cell suspensions from healthy and tumoral tissues obtained from mice of the indicated ...