Transplantation and behavioral recovery of parkinsonian rats.

<p>(A) TH immunohistochemistry of differentiated hMSCs induced by β-mercaptoethanol under 21% O₂ or 3% O₂. Scale bar = 20 µm. (B) The percentage of TH⁺ cells in A. The data are presented as means ± SEM of 6 individual wells. (C) HPLC detection of DA and HVA content in differentiatiated hMSCs induced under 21% O₂ or 3% O₂. The data are presented as means ± SEM of 4 individual wells. (D) Apomorphine-induced rotation from 4 weeks before transplantation to the eighth week after transplantation. <i>n</i> = 7-8 each group. ^#<i>P</i><0.05, <i>versus</i> the rotation at 0 week in the same group. (E) The relative striatal content of DA and its metabolites, DOPAC and HVA, after transplantation. <i>n</i> = 3-5 each group. (F) Cells survival after transplantation were stained using anti-HuN antibody or anti-TH antibody. (G) Cell count of HuN⁺ cells in F. <i>n</i> = 3 each group. (H) Cell count of TH⁺ cells in F. <i>n</i> = 3-4 each group. Data represent mean ± SEM, *<i>P</i><0.05, **<i>P</i><0.01, ***<i>P</i><0.001.