Monocyte apoptosis with regard to bacterial phagocytosis and TNF-α production.

<p>Monocytes were infected with <i>E.coli</i>-GFP for four hours and free bacteria were removed. Four hours p.i. hypodiploid DNA content was quantified and analyzed for absence or presence of intracellular viable bacteria (GFP-negative or positive, respectively), and TNF-α content (A; n = 7, * p<0.05, ** p<0.001). Monocytes were infected with <i>E.coli</i>-EOS-FP for one hour and free bacteria were removed. 4 hours p.i. we quantified hypodiploid DNA content in TNF-α -positive and –negative monocytes in the BI- and P-gate (B; n = 5, * p<0.05, ** p<0.001).