Pattern of LH secretion in a man with complete normosmic CHH due to <i>KISS1R</i> mutations (subject II-1, family 2).

<p>In this individual with very low and nonpulsatile basal LH levels, LH pulsatility was restored by pulsatile GnRH administration. LH secretion was evaluated for 4 hours before GnRH treatment and also on day 16 of pulsatile GnRH administration. <i>Arrows</i> indicate the GnRH boluses and <i>asterisks</i> denote detectable pulses, as analyzed with Thomas' algorithm <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053896#pone.0053896-deRoux1" target="_blank">[7]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053896#pone.0053896-Bouligand1" target="_blank">[27]</a>. Plasma testosterone (T) levels, which were very low before GnRH treatment, increased after pulsatile GnRH administration in this man, indicating testicular stimulation. Conversion to SI units: testosterone, nanograms per milliliter (x 3.467 = nanomoles per liter). The testosterone normal range in men is 2.8–9.0 ng/ml. IB: low serum inhibin B also increased after pulsatile GnRH administration.