Immuno staining of WCR tissues with Anti-ubiquitin.

<p>DsRNA for DvSnf7 (dsDvSnf7) and GFP (dsGFP) were overlaid on diet at 1 µg/ml of diet. Second instar WCR larvae were exposed to dsRNAs for five days. Whole mounts of midgut and fat bodies were used for immuno-staining. Panels A, B, C, D show nuclear DNA (DAPI) staining of midgut and fat bodies. Panels A’, B’, C’, D’ show Alexa-546 detecting ubiquitin. Panels A”, B”, C”, D” show merged images of DAPI and Alexa-546. Panels A*, B*, C*, D* show monochrome images of respective treated tissues at higher magnification. Red arrowheads mark the ubiquitin puncta. Scale Bar: White –50 µm, Red –10 µm.