More mast cells in the peritoneum of Hsd11b1^−/− mice.

<p>(A) Representative dot plots of freshly isolated peritoneal cells (5×10⁵) from <i>Hsd11b1^+/+</i> (left) and <i>Hsd11b1^−/−</i> (middle) mice stained for CD117 (a mast cell marker) or isotype control (right) and subject to flow cytometry. (B) Peritoneal cells from <i>Hsd11b1^−/−</i> mice (−/−, white bars) have more CD117⁺ cells than <i>Hsd11b1^+/+</i> (+/+, black bars), expressed as percentage of total cells (total cell number; <i>Hsd11b1^+/+</i>, 3.8±0.5×10⁶<i>vs Hsd11b1^−/−</i>, 3.7±0.5×10⁶, p>0.05). (C) CD117⁺ cells from <i>Hsd11b1^−/−</i> mice have higher mean fluorescence intensity (MFI) compared to <i>Hsd11b1^+/+</i> mice (black bars). Data are mean ± SEM, n = 8, *p<0.05, **p<0.01.