Lysotracker staining of WCR tissues.

<p>DsRNA for DvSnf7 (dsDvSnf7) and GFP (dsGFP) were overlaid on diet at 1 µg/ml of diet. One set- of second instar WCR larvae were exposed to dsRNAs for four days and then starved for 24 h. Another set of larvae were exposed to dsRNAs continuously for five days. Midgut and fat bodies were dissected from both sets of larvae and used for Lysotracker staining. Panels A–D show Lysotracker and nuclear staining (DAPI) merged images of starved samples. Panels E–H show Lysotracker and nuclear staining (DAPI) merged images of continuously fed samples. Green punctate marks denote Lysotracker staining that detects active acidic lysosomes undergoing autophagy (panels C &D). Scale Bar: 50 µm.