Dependence of the confinement of the E-PSF of CW-STED and gCW-STED recordings on the fluorescence lifetime.

<p>Correlative plot of values pairs of intensity-weighted average lifetime <<i>τ</i>> (<i>i</i> = 3) and FWHM of the intensity profiles through images of individual FNDs. The solid lines show linear regression fits to the experimental data with slopes of −1.99, −0.70 and −0.41 for <i>T_g</i> = 0 ns, 5 ns and 10 ns, respectively. Imaging conditions as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054421#pone-0054421-g003" target="_blank">Figure 3B</a>.