Comparison of the glycosphingolipid binding of classical and El Tor <i>V. cholerae,</i> and an El Tor GbpA deletion mutant strain.

<p>(A, C, E) Chemical detection by anisaldehyde. (B, D, G) Autoradiograms obtained by binding of El Tor <i>V. cholerae</i> strain JBK 70. (F) Autoradiogram obtained by binding of classical <i>V. cholerae</i> strain CVD103. (H) Autoradiogram obtained by binding of El Tor GbpA deletion mutant strain 1382. The lanes on (A and B) were: Lane 1, galactosylceramide (Galβ1Cer), 4 µg, and gangliotriaosylceramide (GalNAcβ4Galβ4Glcβ1Cer), 4 µg; Lane 2, glucosylceramide (Glcβ1Cer), 4 µg, and globotriaosylceramide (Galα4Galββ4Glcβ1Cer), 4 µg; Lane 3, lactosylceramide (Galβ4Glcβ1Cer) with sphingosine and non-hydroxy fatty acids, 4 µg, and Forssman pentaosylceramide (GalNAcα3GalNAcβ3Galα4Galβ4Glcβ1Cer), 4 µg; Lane 4, lactosylceramide (Galβ4Glcβ1Cer) with phytosphingosine and hydroxy fatty acids, 4 µg, and globotetraosylceramide (GalNAcβ3Galα4Galβ4Glcβ1Cer), 4 µg; Lane 5, isoglobotriaosylceramide (Galα3Galβ4Glcβ1Cer), 4 µg, and Le^a pentaosylceramide (Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer), 4 µg; Lane 6, Le^x pentaosylceramide (Galβ4(Fucα3)GlcNAcβ3Galβ4Glcβ1Cer), 4 µg; lane 7, H type 1 pentaosylceramide (Fucα2Galβ3GlcNAcβ3Galβ4Glcβ1Cer), 4 µg. The lanes on (C and D) were: Lane 1, B5 pentaosylceramide (Galα3Galβ4GlcNAcβ3Galβ4Glcβ1Cer), 4 µg; Lane 2, non-acid glycosphingolipids of rabbit erythrocytes, 40 µg; Lane 3, Galα3Galα3Galβ4Glcβ1Cer, 1 µg; Lane 4, B type 2 hexaosylceramide (Galα3(Fucα2)Galβ4GlcNAcβ3Galβ4Glcβ1Cer), 4 µg. The lanes on (E-H) were: Lane 1, non-acid glycosphingolipids of rabbit thymus, 20 µg; Lane 2, lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer), 2 µg; Lane 3, Galα3Galα3Galβ4Glcβ1Cer, 4 µg; Lane 4, B5 pentaosylceramide, 4 µg; Lane 5, gangliotriaosylceramide, 4 µg; Lane 6, H type 1 pentaosylceramide, 4 µg.