Functional validation of pathway inhibitors.

<p>Decidualized St-T1b were preincubated for 1 h with Y27632 (100 µM), NSC23766 (50 µM), Wortmannin (200 nM) (<b>A</b>) or PD98059 (50 µM), LY294002 (10 µM), SB202190 (10 µM) (<b>B</b>), followed by 5 min stimulation with PDGF-BB, TCM or IL-1β. Control cultures received Opti-MEM. Total or phosphorylated (p-) Akt, p38, ERK1/2 and MLC2 were detected by Western blot analysis.