Characterization of C3bot1E174Q-C2I.

<p><i>A.</i> The recombinant fusion toxin C3bot1E174Q-C2I is depicted. <i>B.</i> Coomassie blue staining of GST-C3bot1E174Q-C2I (left lane) and C3bot1E174Q-C2I (right lane) after SDS-PAGE. GST-C3bot1E174Q-C2I was expressed in <i>E. coli</i> and GST was cleaved off with thrombin. <i>C.</i> Western blot analysis of C3bot1E174Q-C2I. C3bot1E174Q-C2I (100 ng) as well as C2I (100 ng) and C3 (100 ng) for control were subjected to SDS-PAGE, blotted and proteins were detected with specific antibodies against C2I and C3bot.