Co-culture with OBs supports hematopoietic stem and progenitor cell function.

<p>7×10³ CD34+ cells were cultured alone or in presence of freshly prepared OBs for 3 and 7 days and the following parameters were measured: (A) Fold increase in total cell number calculated from the original CD34+ cells seeded at day 0. (B) Total CFU output for Control and Co-culture CD34+ cells, cells were plated at day 3 of culture and colonies were scored 14 days after plating; (C) percentage of CD34+CD38- cells; and (D) percentage of CD34+CD38- cell population present in Control and Co-cultured samples at day 7, representative flow cytometry is shown. Absolute numbers of CD34+CD38- cells at day 3 and day 7 of co-culture are shown in table. Values are reported as mean ± 2SEM., * = <i>p</i><0.05 versus Control. Abbreviation: CTR, control.