Survival of EcO157 in dairy wastewater treated with monensin.

<p>EcO157 populations (top panel) were monitored in wastewater treated with (red filled circle) or without (green open circle) monensin. Aerobic bacteria (triangles) and native protozoa (squares) in the presence (red, filled symbols) or absence (green, open symbols) of monensin were also monitored (bottom panel). The monensin-treatment replicate (panel A, red line) with extended survival of EcO157 was chosen for characterization of protozoan and bacterial communities by 18S and 16S rRNA gene sequencing. Corresponding data for total bacteria and protozoa for the replicate are plotted (bottom panel, red lines). Standard deviations for averages of triplicates for untreated wastewater are shown. Replicate differences in survival of EcO157 in monensin-treated wastewater are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054782#pone.0054782.s001" target="_blank">Figure S1</a>.