Detection of <i>LacZ</i> expression in a <i>Nolz-1</i> expressing domain in rostral hindbrain of line #22 UREA-LacZ embryos.

<p>A–C: Expressions of β-gal protein (A), <i>LacZ</i> mRNA (B), and <i>Nolz-1</i> mRNA (C) on coronal hindbrain sections of line#22 E14.5 UREA-LacZ embryo. D–E: High magnification views of the bracketed regions in A–C, respectively. Expressions of <i>LacZ</i> mRNA and β-gal protein are both detected in a differentiated domain of rostral hindbrain (triple arrows in D, E) where endogenous <i>Nolz-1</i> mRNA is expressed (F). <i>LacZ</i> expression is, however, absent in the nearby Nolz-1-expressing ventricular zone (vz, F). Double arrows in A and B indicate a domain in which β-gal expression is detected with little <i>LacZ</i> mRNA expression. IV, fourth ventricle; mb, midbrain.