Dietary supplementation with combined fish oil and selenium yeast reduces immunosuppressive activity of spleen cells in tumor-bearing animals on CD8⁺ CTL and NK cells activities.

<p>A, Winn assays were used to quantify inhibitory effects of splenocytes from tumor-bearing mice (n = 9–10 mice/group from two independent experiments) on endogenous CD8⁺ T cell activity. B, Dietary supplementation with fish oil and selenium yeast was shown to enhance the CD8⁺ CTL activity even after depletion of CD4− and CD8− specific T cells in splenocytes. Data show n = 9–10 mice/group, and mean ± SD. is representative of results from two independent experiments. C, Tumors in mice were intervened with or without addition of fish oil and selenium yeast. After sacrifice, splenocytes from BALB/cByJ mice were used as the effector cells to test the protective effect of experimental diets for the NK-associated cytotoxicity against target cells (line-1). Data show mean ± SD. of n = 7–8 mice/group and are representative of results from three independent experiments. *p<0.05, and **p<0.01 denote levels of significant differences between groups.