AngII promotes binding of c-Myc to the AdipoR1 promoter region and it requires ERK1/2 involvement.

<p>(A) NRVMs were stimulated with 0.1 µmol/L AngII for 48 h. Sheared chromatin prepared from cardiomyocytes was immunoprecipitated with the indicated antibodies (control IgG, anti-c-Myc, anti-STAT5 or anti-NF-κB antibodies). The immunoprecipitated DNA was used as a template for PCR using the indicated primer sets. (B) NRVMs were pretreated with 10 µmol/L PD98059 for 1 h, then stimulated with 0.1 µmol/L AngII for 48 h. Chromatin fragments were immunoprecipitated with anti-c-Myc antibody or normal rabbit IgG. Immunoprecipitated chromatin was quantified with RT-PCR. (C) NRVMs were pretreated with losartan (10 µmol/L, lane 3), NAC (10 mmol/L, lane 4), apocynin (100 µmol/L, lane 5), rotenone (10 µmol/L, lane 6), gp91 ds-tat (10 µmol/L, lane 7) or PD98059 (10 µmol/L, lane 8) for 1 h, and then stimulated with 0.1 µmol/L AngII for 48 h. DNA binding activity of c-Myc was determined by EMSA with nuclear extracts of NRVMs and DNA probes. Lane 1, control; lane 2, AngII; lane 3–8, inhibitors; lane 9, with 100-fold molar excess of unlabeled oligonucleotide; lane 10, with 100-fold molar excess of unlabeled mutant oligonucleotide.