Clinical samples analyzed by PCR for <i>M. ulcerans</i>.

<p><a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002011#pntd-0002011-t004" target="_blank">Table 4</a> indicates all samples tested by PCR at IHN and DITM. During the initial phase (phase I) samples were analyzed by standard gel-based IS<i>2404</i> PCR at DITM. During the second phase (phase II) parallel samples were subjected to IS<i>2404</i> dry-reagent based (DRB) PCR at INH and standard IS<i>2404</i> PCR at DITM. During both phases all samples tested negative in standard PCR were subjected to re-testing by IS<i>2404</i> quantitative real-time PCR (qPCR) at DITM.</p>a<p>Swab, DNA extracts prepared from swab samples.</p>b<p>FNA, DNA extracts prepared from fine-needle aspirate samples.</p>c<p>Punch, DNA extracts prepared from 3 mm punch biopsy samples.</p>d<p>Phase I, initial phase of implementation of the national reference laboratory at INH from September through December 2010.</p>e<p>Only samples tested negative in standard IS<i>2404</i> PCR were subjected to IS<i>2404</i> qPCR at DITM.</p>f<p>Total amount of samples tested by DRB- and Standard PCR during the corresponding phases.</p>g<p>Phase II, transitional phase of implementation of the national reference laboratory at INH from January 2011 through April 2012.