Curing of [<i>PSI</i>⁺] by Pin4C depends on cell division.

<p>Three <i>MAT</i>a strong [<i>PSI</i>⁺][<i>PIN</i>⁺] <i>SUP35-GFP</i> (GF845) transformants harboring pHR81<i>GAL-PIN4C</i> were grown in liquid galactose media for 40 hrs, when Sup35-GFP foci became larger in size and fewer in number. Then cells were transferred to fresh galactose medium with or without the addition of 50 µM a-factor for another 16 hrs. Three transformants harboring empty vector pHR81-<i>GAL</i> were transferred to galactose medium without the addition of 50 µM a-factor as control. Samples were taken, diluted and plated on YPD where the percentage of red cured [<i>PSI</i>⁺] among ∼850 colonies was scored (black bars). There was no [<i>PSI</i>⁺] loss in the control. The number of CFUs is shown by gray bars. Error bars show standard error of the mean.