Percentage of biotinylated flagellar protein bands recovered in flagellar vesicle fractions after 6 hr incubation.

<p>Densitometric analysis of biotinylated bands in detergent extracts (membrane+matrix) of purified flagella and released flagellar vesicles. Biotinylated cells were incubated for 6 hrs. Cells were pelleted, deflagellated, and flagella were purified and extracted with NP-40. Vesicles were purified from the culture medium. Membrane+matrix and vesicle polypeptides were fractionated by SDS page and biotinylated proteins were stained on western blots. The densities of eight bands (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053366#pone.0053366.s003" target="_blank">Fig. S3</a>) were measured and the proportion of polypeptides in the vesicle fraction compared to that in each membrane+matrix was determined based on the percentage of the sample loaded on the gels. Measurements from 5 different experiments and the average recovered protein for each of the bands are shown. The amount of FV protein recovered varied with different bands. A value of 100% indicates that the FV contains 100% of the biotinylated protein present in each flagellum. During the incubation, flagella maintain their quantities of biotinylated protein by drawing on the plasma membrane pool (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053366#pone-0053366-g003" target="_blank">Fig. 3A</a>).