Two-photon time lapse imaging of GTTR uptake into live <i>Cdh23^v2J</i> transgenic mouse cochlear hair cells.

<p>P3 cochleae of mouse litters from <i>Cdh23^v2J/+</i> breeding were isolated and cultured overnight before treatment with GTTR (3 µM×1 hr). A–C) GTTR rapidly entered into outer hair cells of wildtype and <i>Cdh23^v2J</i>^/+ cochleae, whereas GTTR did not enter hair cells of <i>Cdh23^v2J</i>^/v2J cochleae. Insets depict magnified views of outer hair cells of cochleae of each genotype after exposure to GTTR for 60 min. D) Quantification of fluorescence intensity (AU = arbitrary units) shows that the rate of GTTR uptake among outer hair cells from wildtype and heterozygous mice were comparable, while those from homozygous mice had no detectable drug uptake. Error bars = S.D., scale bar = 20 µm in A–C and 5 µm in insets.