Targeted mutagenesis in primary cells and iPS.

<p>Cells were transfected with the CAPNS1m meganuclease in the absence (empty) or presence of DNA-end processing enzymes; scTrex, single-chain Trex2; scTrex-CAPNS1, meganuclease fused to scTrex; Tdt, terminal deoxynucleotidyltransferase. Frequency of TM as determined by sequence analysis of locus specific amplicons; ns, not significant. (A) Frequency of TM measured in Detroit 551 human fetal fibroblasts in 2 independent experiments. (B) Frequency of TM measured in iPS in 2 independent experiments.