Bone marrow and spleen erythroid parameters.

<p>Control (Ctrl) and hemin (HE)-treated mice were euthanized 24 h after the last injection with PBS or HE. Populations of erythroid cells were analyzed in the bone marrow (A) and in the spleen (B) by staining cells with anti-CD71-FITC and anti-Ter119-PE antibodies. The percentage of reticulocytes (CD71⁺ cells) (C) and hemoglobin levels were measured in the blood (D). Macrophages (F4-80⁺ cells) were quantified in femoral bone marrow cell suspensions (E) by staining with anti-F4/80-PE antibody. Erythropoietin (EPO) was measured in the plasma by ELISA (F). Data are mean ± SEM from two independent experiments (n = 4–11 mice per group) and values were compared using a non-parametric Student <i>t</i> test. <i>*p<</i>0.05; **<i>p</i><0.01; ***P<0.001.