SB225002 induces mitotic catastrophe.

<p>A: Time-dependent induction of mitosis (filled circles) and subsequently apoptosis (filled squares) in OV2008 cells by SB225002. B: 24 h after SB225002 treatment, mitotic spindles were visualized with an Alexa Fluor® conjugated to anti-α-tubulin (green) and counterstained with Hoechst (blue). In control cells, normal bipolar spindle was well organized with highly condensed chromosomes aligned along the equatorial plates during meta-phase and sister chromatids segregated at the onset of anaphase (B-1). SB225002-treated cells displayed mitotic aberrations. The frequencies of lagging chromosomal material, multipolar mitotic spindles and multiple nuclei were all increased in SB225002-treated cells (B-2, B-3). C: Chromosome spreads showing SB225002-induced aneuploidy/polyploidy.