Construction of <i>fimH</i> chromosomal mutants.
- Nicolas Dreux (277061)
- Jérémy Denizot (209615)
- Margarita Martinez-Medina (277062)
- Alexander Mellmann (72273)
- Maria Billig (277063)
- Dagmara Kisiela (277064)
- Sujay Chattopadhyay (158532)
- Evgeni Sokurenko (277065)
- Christel Neut (241629)
- Corinne Gower-Rousseau (277066)
- Jean-Frédéric Colombel (108372)
- Richard Bonnet (241637)
- Arlette Darfeuille-Michaud (112082)
- Nicolas Barnich (277067)
Publication date
January 2013
DOIAbstract
<p>(A) Construction of <i>fimH</i> chromosomal mutants. (B) Electron microscopy examination of AIEC LF82 bacteria, LF82-Δ<i>fimH</i> isogenic mutant, LF82-Δ<i>fimH/fimH<sub>LF82</sub></i>, LF82-Δ<i>fimH/fimH<sub>K12</sub></i>, LF82-Δ<i>fimH/fimH<sub>7082</sub></i>, LF82-Δ<i>fimH/fimH<sub>LF28</sub></i>, LF82-Δ<i>fimH/fimH<sub>LF16</sub></i>, LF82-Δ<i>fimH/fimH<sub>7049</sub></i>, LF82-Δ<i>fimH/fimH<sub>LF31</sub></i>, LF82-Δ<i>fimH/fimH<sub>7136;</sub></i> LF82-Δ<i>fimH/fimH<sub>LF73</sub></i> showing flagella and type 1 pili expression (magnification ×40 000). (C) Yeast agglutination titer of AIEC LF82 bacteria, LF82-Δ <i>fimH</i> isogenic mutant and <i>fimH</i> chromosomal mutants.</p
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