The T6SS is active in several species of <i>Acinetobacter</i>.

<p> A) Whole cell and supernatant samples prepared from cultures of several <i>A. baumannii</i> strains were probed with anti-Hcp (top panels) and the lysis control anti-RNA polymerase (RNAP; bottom panels). B) Whole cell and supernatant samples prepared from cultures of different species within the genus <i>Acinetobacter</i> probed as described above. C) Summary of growth and Hcp secretion characteristics, determined by Western blot and ELISA, of all T6SS-positive strains analyzed in this study. “Fast” growing strains (++) and “slow” growing strains (+) were defined as those which reached a high or low optical density, respectively, and set arbitrarily by the indicated line in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055142#pone.0055142.s002" target="_blank">Figure S2</a>. Hcp secretion is summarized as high (↑) or low (↓) based on Western blots and ELISA assays (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055142#pone.0055142.s001" target="_blank">Figure S1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055142#pone.0055142.s002" target="_blank">S2</a>).