Tc differential response to Cry3Ba and Cry23Aa/Cry37Aa spore-crystal treatments.

<p>(<b>A</b>) Relative levels of Tc pyruvate dehydrogenase (Pdh), cuticular protein and ribosomal protein L13a (RPL13a) mRNAs normalized to the corresponding untreated control, determined by qRT-PCR analysis in control larvae and larvae exposed to Cry3Ba and Cry23Aa/Cry37Aa spore-crystal mixtures, using RPS18 mRNA as reference. Error bars indicate standard errors of the means from two biological replicates of twenty-four individuals per replicate. (<b>B</b>) Monitoring of Tc larvae development following Cry3Ba and Cry23Aa/Cry37Aa spore-crystal treatments that produced 15–20% mortality. Percentage of pupae was recorded after seven days of spore-crystal treatment and up to day 35, once surviving treated larvae were transferred to spore-crystal-free diet.