Microarray analysis of 4HT, endoxifen and ICI action in the absence of estrogen.

<p>(<b>A</b>) Venn diagram of genes whose expression levels were determined to be significantly altered by 100 nM concentrations of 4HT, endoxifen or ICI in the absence of estrogen following 24 hours of regardless of fold-change levels. (<b>B</b>) A second Venn diagram was developed using only those genes which exhibited fold-changes of 1.4 fold or greater relative to vehicle treated control cells. (<b>C</b>) Heat map depicting the hierarchical clustering of genes that were differentially expressed in at least one of the indicated treatment groups relative to vehicle control and which had average fold-changes >3 standard deviations from all other genes in the comparison. Red indicates increased gene expression while green indicates decreased gene expression relative to vehicle treated controls.