Dynamic properties of neuroblast migration in ischemic areas.

<p><b><i>A</i></b>–<b><i>F</i></b>, Timeline snapshots from real-time images of SVZ-derived precursor migration in acute brain slices of MCAo-challenged animals. Cells were labeled by injecting GFP-encoding retroviruses in the SVZ prior to inducing ischemia and were evaluated 2 to 3 weeks post-MCAo in the contralateral RMS (<b><i>A</i></b>,<b><i>B</i></b>), ipsilateral RMS (<b><i>C</i></b>,<b><i>D</i></b>), and ipsilateral striatum (<b><i>E,F</i></b>). Arrowheads indicate migrating cells. <b><i>B</i></b>,<b><i>D</i></b>,<b><i>F,</i></b> Migratory tracks of the cells migrating for 1 h in <b><i>A,C,E</i></b>, respectively (color code: blue in the initial position; white in the final position). <b><i>G</i></b>, Representative profile of cell displacement over time for individual neuroblasts migrating in the contralateral and ipsilateral RMS and the striatum. <b><i>H</i></b><b>,</b><b><i>J,</i></b> In the ipsilateral striatum, the recruited neuronal precursors displayed low migratory behavior, spending longer periods in the resting state (<b><i>J</i></b>), which resulted in shorter displacements per hour compared to the contralateral or ipsilateral RMS (<b><i>H</i></b>). <b><i>I</i></b>, No differences in the speed of migration of neuroblasts migrating in the ischemic striatum and ipsilateral and contralateral RMS were observed. Scale bars: 20 µm (CL: contralateral; IPSI: ipsilateral; STR: striatum).