Fixation and permeabilization of human islets does not affect the content of miRNAs.
- Dagmar Klein (277684)
- Ryosuke Misawa (277685)
- Valia Bravo-Egana (277686)
- Nancy Vargas (211230)
- Samuel Rosero (277687)
- Julieta Piroso (277688)
- Hirohito Ichii (277689)
- Oliver Umland (277690)
- Jiang Zhijie (277691)
- Nicholas Tsinoremas (277692)
- Camillo Ricordi (51444)
- Luca Inverardi (211256)
- Juan Domínguez-Bendala (211258)
- Ricardo L. Pastori (211257)
Publication date
January 2013
DOIAbstract
<p>Human islets were fixed and permeabilized as they were for FACS analysis. miRNAs expression was determined by qRT-PCR and fold changes (treated vs untreated islets) were expressed as mean +/− SD (n = 4). Control bar representing untreated islets is equal 1 also marked by the red dotted line. Paired, two-tail t test revealed no statistically significant differences in miRNAs expression (p>0.05).</p
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