PKCs contribute to PLCγ-dependent microtubule capture.

<p>(<b>A</b>) SKBr3 cells expressing EGFP-tubulin and APC or membrane-targeted ACF7 (ACF-CCKVL) were treated with PKC inhibitory peptides, before addition of HRG and determination of the percentage of cells displaying peripheral microtubules by live fluorescence microscopy. Expression of APC or ACF7-CCKVL only rescued microtubules of cPKC-inhibited and not of aPKC-inhibited cells. (<b>B</b>) SKBr3 cells expressing EGFP-tubulin were transfected with control (Ctrl) or PLCγ siRNA and constitutively active PKCα (myr-PKCα) or PKCζ (myrPKC)ζ, before addition of HRG and analysis by live fluorescence microscopy. Percentages of cells with microtubules captured at the leading edge and cells with microtubules stabilized at a short distance of the leading edge were determined. 90–150 cells were counted per condition in three independent experiments; mean+/−s.e.m. is shown; * p<0.01 relative to the respective controls.