- Annexin V-FITC binding in <i>L. (L.) amazonensis</i>.

<p>(A) Bar diagram of the percentage of annexin V-FITC positive population calculated as explained in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055604#pone-0055604-g003" target="_blank">fig 3</a>. Log-phase (Log), stationary-phase (Stat) and metacyclics-enriched (Met) promastigotes left untreated (control) or treated with 40 µM ionomycin (iono) were labeled for 20 min at 2°C with annexin V-FITC and PI, and subsequently analyzed by flow cytometry or microscopy. For calcium depletion, BAPTA-AM and EGTA were added for 30 min at 2°C before adding ionomycin (BAPTA/EGTA/iono). Results represent the mean ± SD of two independent experiments. Significant differences were determined by Student paired t-tests and denoted by asterisks (P<0.05). (B) Annexin V-FITC staining (green) combined with phase-contrast micrographs are depicted. Control and ionomycin treatments were as described in A. The bars indicate 5 µm.