Differential localization of PABP1 and PABP2 to inducible RNP granules.

<p>Z-stack projections of fluorescent microscopy images of cells coexpressing PABP1-eYFP and PAPB2-mChFP, PABP1-eYFP and mChFP-DHH1 or PABP2-eYFP and mChFP-DHH1 are shown. Cells were either untreated or treated with nutrient starvation (2 h PBS), heat shock or sinefungin. The arrows point to heat shock induced PABP1 and PABP2 granules that colocalize. Note that for the heat shock experiment of the cell line expressing PABP1 and DHH1 fusions a cell line was used expressing PABP1-mChFP and eYFP-DHH1; images are shown in false colors for clarity.