Repression of gene expression in splenocytes and pancreata by TSA treatment.

<p>(<b>A</b>) Total RNA was extracted from splenocytes of 24–28 wk old overtly diabetic mice and 28–34 wk old TSA-treated and cured mice that were different from those used for microarray analysis. RNA was converted into cDNA and used for validation of microarray data by qRT-PCR. (<b>B</b>) Expression of the same set of genes was determined by using RNA isolated from pancreata of diabetic and cured mice. Mean +/− SD of triplicate determinations from a representative of 2–3 experiments are shown. In each experiment, RNA was pooled from 3–5 mice. P values between groups are indicated. NS, not significant.