βKlotho overexpression inhibited hepatoma cell proliferation.

<p>(A) Colony formation assay. Representative micrographs (left panel) and quantification (right panel) of crystal violet-stained Hep3B or SMMC-7721 cells transfected with either vector or βKlotho. (B) Viability of βKlotho-transfected or vector-transfected Hep3B cells were determined by MTT assay on days 1 to 5 after transfection. (C) Viability of βKlotho-transfected or vector-transfected SMMC-7721 cells were determined by MTT assay on days 1 to 5 after transfection. Each bar represents the average ± SD of three independent experiments. * indicates <i>p</i> < 0.05.