Expression of TRPM4 channel in whole cell extracts and plasma membrane fraction.

<p>Several TRPM4 mutants show an alteration of protein expression at a total level as well as at the cell surface. (<b>A</b>) Whole cell lysates from HEK-293 cells transfected with TRPM4 constructs used as input, representing the total expression of TRPM4 protein. Quantification of the double bands, presumably fully and core glycosylated forms of TRPM4, black and white arrrows, respectively, is shown on the bottom panels. (<b>B</b>) The biotinylated fractions from the same transfection represent the amount of TRPM4 expressed at the cell surface. Quantification of the double bands is shown on the bottom panels. n = 3. *p<0.05, **p<0.01, ***p<0.001. Error bar: standard error of the mean.