Differential proteome analyses depending on hydrostatic pressure.

<p>Proteome profiles of <i>D. piezophilus</i>. <b>A.</b> Overview of the proteome profile obtained by 2DE-gels of soluble proteins recovered from <i>D. piezophilus</i> grown at 10 MPa. The proteins (100 µg) were separated by 2D gel electrophoresis on a pH gradient of 4–7 and a 10–18% polyacrylamide denaturing gradient, and the gel was silver-stained. A total of 727 protein spots were detected. <b>B.</b> Overlapping DiGE images of the <i>D. piezophilus</i> proteome. The proteins were separated on a pH gradient of 3–10 and a 12% polyacrylamide denaturing gel (Cy3-green = 10 MPa; Cy5-red = 0.1 Mpa). A total of 940 protein spots were detected. <b>C.</b> Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the membrane protein fraction of <i>D. piezophilus</i> recovered at low (0.1 MPa, Lane 1) or high (10 MPa, Lane 2) hydrostatic pressure. Molecular weight standard, MW. The arrows indicate the positions of the differentially abundant proteins according to the hydrostatic pressure growth conditions.