Effect of pH and temperature on the activity and stability of rMxyl.

<p><b>A</b> and <b>B:</b> The recombinant xylanase incubated in various buffers (pH 3–12) and temperatures (40–100°C) and assayed for xylanase activity <b>C:</b> Recombinant xylanase was incubated in glycine-NaOH buffer without substrate and kept at various temperatures. Aliquots were collected at various time interval and store at 0°C for calculating residual activity. <b>D:</b> Similarly enzyme was incubated in various buffers (pH 8–11) and aliquots of different time intervals were used xylanase assays.