LiCl attenuates FBS-induced VSMC proliferation and migration.

<p>VSMCs were infected with recombinant adenoviruses expressing PGC-1α siRNA or scrambled siRNA for 30 hr and then treated as previously described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055471#pone-0055471-g001" target="_blank">Fig. 1</a>. Cell proliferation was evaluated by MTT (A) and Edu (B) assays. The scale bar indicates 50 µm. For the determination of cell migration, transwell (C) and wound healing (D) assays were used. Migrated cells were quantified by the average of 5 randomly chosen high-power fields (HPF) of 3 independent duplicate experiments. The scale bar indicates 50 µm (C) or 200 µm (D). **<i>P</i><0.01 compared with the control group; ⁺<i>P</i><0.05, ⁺⁺<i>P</i><0.01 compared with the serum-treated group; ^##<i>P</i><0.01 compared with the control siRNA group.