LiCl blocks FBS-induced activation of signaling cascades.

<p>VSMCs were treated as previously described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055471#pone-0055471-g002" target="_blank">Fig. 2</a> and the cell lysates were subjected to Western Blot analysis (A). Note: for the detection of kinase phosphorylation, FBS stimulation time was shortened to 30 min. Measurement of NO production (B). Data are presented as means ± SEM from three independent experiments. **<i>P</i><0.01 compared with the control group; ⁺⁺<i>P</i><0.01 compared with the serum-treated group; ^#<i>P</i><0.05 compared with the control siRNA group.