Growth curve inhibition as assessed by MTT assay in IMR-32, TGW, SH-SY5Y and Neuro-2a cells (a).

<p>Data are representative of three different experiments done in triplicates and expressed as mean ± S.E.M. (b) Phase contrast images of IMR-32, TGW, SH-SY5Y and Neuro-2a neurolastoma cells treated with 0.0% (Control), 0.2% and 0.5% ASH-WEX. (c) NF200 expression in response to ASH-WEX treatment in control, ASH-WEX (0.2% and 0.5%) and RA treated IMR-32 cultures. The relative intensity measurement of immunofluorescence is shown (d). (e) Representative Western blot hybridization signals for NF200 from control and test samples. (f) Representative RT-PCR results for NF200 mRNA in control and treated cells and their relative densitometry analysis represented by histograms. “*” represents the statistical significant (p<0.05) difference between control and ASH-WEX treated groups.