Diminished proportions of HLA-G⁺ CD4 and CD8 T cells in progressive HIV-1 infection.

<p>(A): Representative flow cytometry dot plots reflecting the proportions of HLA-G⁺ CD4 and CD8 T cells in indicated study subjects. FMO control reflects “fluorescence minus one” control condition without addition of HLA-G antibodies. (B): Box and Whisker plots summarizing the relative proportions and absolute numbers of HLA-G⁺ CD4 and CD8 T cells in indicated study cohorts. ANOVA followed by post-hoc analysis with Tukey's Multiple Comparison Test was used to determine significance. (C): Correlations between frequencies of HLA-G⁺ T cells and total CD4 T cell counts in controllers (n = 23), progressors (n = 27) and HIV seronegative individuals (n = 15). (D): Correlations between proportions of HLA-G⁺ Treg and CD8 T cell immune activation determined by surface expression of CD38 and HLA-DR in controllers (n = 19), progressors (n = 20), and HIV seronegative individuals (n = 15). (C/D): Spearman's correlation coefficient is shown.