Time dependent transcription profiles of the cytosolic thioredoxin system in culture-derived and mosquito-derived parasites.

<p>RT-qPCR data showing relative target gene expression as fold increase over time. The 3-hour time point was used as a reference. All data was normalized against the expression of 18 s rRNA A-type <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003136#ppat.1003136-Yano1" target="_blank">[29]</a>, <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003136#ppat.1003136-Thompson1" target="_blank">[34]</a>. The Mann-Whitney U test was conducted on each candidate gene from both mosquito-derived and from culture-derived parasites. Significance was assessed at p<0.1(*) due to the small sample sizes. Shown are mean values of 4 independent blood feeding experiments (n = 55 mosquitoes/time point/experiment) and 3 independent ookinete culture setups, respectively. Error bars show STDEV.